Journal: Acta ophthalmologica

Article Title: Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing.

doi: 10.1111/j.1755-3768.2012.02388.x

Figure Lengend Snippet: Fig. 1. Corneal re-epithelialization after alkali wounding is accelerated by treatment with bone marrow (BM) cells or CD117+ cells. Photographs show representative fluorescein-stained cor- neal wounds of control, BM and CD117 group at different time-points during healing process. The edges of the wound areas are marked with white dotted lines that were quantified using the software sigma scan pro 5.0.

Article Snippet: Magnetic-activated cell sorting to isolate CD117+ cells Part of the isolated BM cells was resuspended with magnetic-activated cell sorting (MACS ) buffer [PBS pH 7.2, 0.5% FCS (Invitrogen) and 2 mMol EDTA (Merck, Darmstadt, Germany)] at a concentration of 107 cells per 80 ll and incubated with CD117 MicroBeads (Miltenyi Biotec).

Techniques: Staining, Control, Software

Journal: Acta ophthalmologica

Article Title: Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing.

doi: 10.1111/j.1755-3768.2012.02388.x

Figure Lengend Snippet: Fig. 2. Bone marrow (BM) cells or CD117+ cells accelerate the corneal wound healing follow- ing alkali burn injury. Kaplan–Meier curves (A) show the probability of corneal wound healing at different time-points in control, BM and CD117+ group. Censored cases are indicated by small vertical tick marks. Corresponding to the curves, the number of remaining corneal wounds in each group during treatment and follow-up interval are listed in the table below (B). Corneal wound healing is significantly enhanced by local application of BM cells or CD117+

Article Snippet: Magnetic-activated cell sorting to isolate CD117+ cells Part of the isolated BM cells was resuspended with magnetic-activated cell sorting (MACS ) buffer [PBS pH 7.2, 0.5% FCS (Invitrogen) and 2 mMol EDTA (Merck, Darmstadt, Germany)] at a concentration of 107 cells per 80 ll and incubated with CD117 MicroBeads (Miltenyi Biotec).

Techniques: Control

Journal: Acta ophthalmologica

Article Title: Bone marrow cells and CD117-positive haematopoietic stem cells promote corneal wound healing.

doi: 10.1111/j.1755-3768.2012.02388.x

Figure Lengend Snippet: Fig. 3. Cells covering the wounded area are corneal epithelial cells. Images show histological and immunohistological cross-sections through the corneal wound area after re-epithelialization for control (B,F,J,N), bone marrow (BM) (C,G,K,O) and CD117 (D,H,L,P) group. For com- parison, unwounded healthy cornea (normal) was also examined (A,E,I,M). Haematoxylin and eosin staining (HE) show no apparent differences between healed corneas of control, BM and CD117 group (B,C,D). Unwounded healthy cornea consists of five to seven epithelial cell layers, whereas corneas of control, BM and CD117 group compose two to three cell layers. Immunolo- calization of cytokeratin 3 ⁄ 12, a specific marker for corneal epithelial cells, demonstrates no substantial quantitative difference in the corneal epithelium of control, BM and CD117 group (F–H). The multilayered epithelium in normal cornea is evident (E). To detect any signal with the fluorescence microscope, all images with the cell tracer carboxyfluorescein diacetate succin- imidyl ester (CFDA-SE) were overexposed (I–L). A specific fluorescence of the dye was not detectable in the cells. Immunohistochemistry of all corneas is negative for CD117 Ag (M–P). The nuclei in these sections are counterstained with 4¢,6-diamidino-2-phenylindole (blue). The position of the Bowman¢s membrane is marked by arrows. In addition, Bowman¢s membrane and Descemet¢s membrane are highlighted by white lines. Star: lens or iris tissue.

Article Snippet: Magnetic-activated cell sorting to isolate CD117+ cells Part of the isolated BM cells was resuspended with magnetic-activated cell sorting (MACS ) buffer [PBS pH 7.2, 0.5% FCS (Invitrogen) and 2 mMol EDTA (Merck, Darmstadt, Germany)] at a concentration of 107 cells per 80 ll and incubated with CD117 MicroBeads (Miltenyi Biotec).

Techniques: Control, Staining, Marker, Microscopy, Immunohistochemistry, Membrane

Journal: Biomicrofluidics

Article Title: A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

doi: 10.1063/1.4941984

Figure Lengend Snippet: Finite element simulation result of the pressure profile of the immobilization channel with one worm loaded and another worm at the channel inlet. The simulation was performed in ANSYS 13.0 (Canonsburg, USA). The pressure drop along the worm trapped at the inlet of the immobilization channel is too small to push the animal into the immobilization channel. The 3D model of the immobilization channel and worm and the associated mesh were developed by the ANSYS Workbench 13.0. The worm body was represented by a cylinder with cone-shaped head and tail. Incompressible steady-state Navier-Stokes equations and no-slip boundary conditions were used in the simulation. The pressure difference between the inlet and outlet of the channel was set to be 30 kPa.

Article Snippet: The 3D model of the immobilization channel and worm and the associated mesh were developed by the ANSYS Workbench 13.0.

Techniques: